|
| Status |
Public on Nov 08, 2023 |
| Title |
Real-time quantitative PCR analysis of human HepaRG cells infected with hepatitis E virus (HEV) [D100 plaque 1] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during chronic HEV infection in human hepatocytes, the main site of HEV replication, using HepaRG cells.
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| |
|
| Overall design |
HepaRG cells infected with HEV-3f at a MOI of 100 GE/cell and their matched non-infected controls were analyzed at 100 days post-infection. Triplicate samples from 2 independant experiments were analyzed.
|
| |
|
| Contributor(s) |
Doceul V, Meyer L, Duquenois I |
| Citation(s) |
38058490 |
| Submission date |
Sep 22, 2023 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Virginie Doceul |
| E-mail(s) |
virginie.doceul@vet-alfort.fr
|
| Organization name |
UMR VIROLOGIE INRAE-ANSES-ENVA
|
| Street address |
7 avenue du Général de Gaulle
|
| City |
Maisons-Alfort |
| ZIP/Postal code |
94700 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL33776 |
RT² Profiler PCR Array Human Type I Interferon Response (custom) |
|
| Samples (12)
|
| GSM7797007 |
Non-treated HepaRG (SAMPLE 1), D100 plaque 1 |
| GSM7797008 |
Non-treated HepaRG (SAMPLE 2), D100 plaque 1 |
| GSM7797009 |
Non-treated HepaRG (SAMPLE 3), D100 plaque 1 |
| GSM7797010 |
HEV-infected HepaRG (SAMPLE 4), D100 plaque 1 |
| GSM7797011 |
HEV-infected HepaRG (SAMPLE 5), D100 plaque 1 |
| GSM7797012 |
HEV-infected HepaRG (SAMPLE 6), D100 plaque 1 |
| GSM7797013 |
Non-treated HepaRG (SAMPLE 7), D100 plaque 1 |
| GSM7797014 |
Non-treated HepaRG (SAMPLE 8), D100 plaque 1 |
| GSM7797015 |
Non-treated HepaRG (SAMPLE 9), D100 plaque 1 |
| GSM7797016 |
HEV-infected HepaRG (SAMPLE 10), D100 plaque 1 |
| GSM7797017 |
HEV-infected HepaRG (SAMPLE 11), D100 plaque 1 |
| GSM7797018 |
HEV-infected HepaRG (SAMPLE 12), D100 plaque 1 |
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| This SubSeries is part of SuperSeries: |
| GSE243864 |
Real-time quantitative PCR analysis of liver tissue from pigs infected experimentally with hepatitis E virus (HEV) |
|
| Relations |
| BioProject |
PRJNA1020176 |
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