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| Status |
Public on Apr 08, 2024 |
| Title |
Bulk RNA-Seq of actinic keratoses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq was performed on 8 actinic keratoses (AK) to identify differentially expressed genes in AK vs normal skin and cutaneous squamous cell carcinoma
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| Overall design |
Total RNA was then isolated from actinic keratoses using QIAshredder (Qiagen) and Trizol (ThermoFisher) followed by DNA removal with the TURBO DNA-free ki t (Ambion) according to the manufacturer’s instructions. RNA integrity was verified using an Agilent 2100 Bioanalyzer. RNA-Seq libraries were prepared with the mRNA Seq Sample Prep K it (Illumina, Inc.) as recommended by the manufacturer. 101 bp paired-end sequencing reads were obtained using the Illumina HiSeq platform.
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| |
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| Contributor(s) |
Tsai KY, Bencomo TJ, Rajapakshe K, Coarfa C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 14, 2024 |
| Last update date |
Apr 08, 2024 |
| Contact name |
Carolyn Lee |
| E-mail(s) |
carilee@stanford.edu
|
| Organization name |
Stanford University School of Medicine
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1088157 |
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