|
| Status |
Public on Apr 08, 2024 |
| Title |
P45_AK |
| Sample type |
SRA |
| |
|
| Source name |
Skin
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skin
disease state: Actinic Keratosis
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
TotalRNAwasthenisolatedfromhumantissuesusingQIAshredder(Qiagen)andTrizol(ThermoFisher)followedbyDNAremovalwiththeTURBODNA-freekit(Ambion)accordingtothemanufacturer’sinstructions.
RNA-Seq libraries were prepared with the mRNA Seq Sample Prep Kit (Illumina, Inc.) as recommended by the manufacturer.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
P45_AK
|
| Data processing |
Sampleswerepseudoalignedandquantifiedtothehg38referencegenomeusingSalmon(version1.5.1)togeneratecountmatrices
Assembly: hg38
Supplementary files format and content: Tab-delimited text files containing transcript by sample count matrix
|
| |
|
| Submission date |
Mar 14, 2024 |
| Last update date |
Apr 08, 2024 |
| Contact name |
Carolyn Lee |
| E-mail(s) |
carilee@stanford.edu
|
| Organization name |
Stanford University School of Medicine
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN40463079 |
| SRA |
SRX23954954 |
