|
| Status |
Public on May 01, 2024 |
| Title |
CD40 stimulation + dasatinib treatment of chronic lymphocytic leukemia (CLL) cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this experiment we in vitro activated CLL cells on a layer of fibroblasts expressing CD40L (3T40) in the presence of 100 nM Dasatinib for 48 hours. After the 48 hours, cells were taken off the fibroblasts and sorted for viable CD19+ cells. Then we performed RNA sequencing.
|
| |
|
| Overall design |
This experiment was analyzed together with another GEO submission (GSE201341), the comparison was between 3T40 and 3T40 + DAS conditions.
|
| |
|
| Contributor(s) |
Peters FS, van Bruggen JA, Kater AP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 22, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Fleur Peters |
| Organization name |
Amsterdam UMC (AMC)
|
| Street address |
Meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (3) |
| GSM8222574 |
CLL cells from patient 1 cultured on 3T40 for 48 hours in presence of 100 nM Dasatinib |
| GSM8222575 |
CLL cells from patient 2 cultured on 3T40 for 48 hours in presence of 100 nM Dasatinib |
| GSM8222576 |
CLL cells from patient 4 cultured on 3T40 for 48 hours in presence of 100 nM Dasatinib |
|
| Relations |
| BioProject |
PRJNA1103147 |
