|
| Status |
Public on May 01, 2024 |
| Title |
CLL cells from patient 2 cultured on 3T40 for 48 hours in presence of 100 nM Dasatinib |
| Sample type |
SRA |
| |
|
| Source name |
Blood PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood PBMCs
cell type: B cells
Sex: Female
mutation status: Unmutated
time: 48 hours
treatment: 3T40+dasatinib
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiagen Mini kit
KAPA mRNA Hyperprep
mRNA sequencing on Illumina HiSeq 4000
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Pt2_T48_3T40DAS
|
| Data processing |
Sequence quality was assessed with FastQC, raw FASTq files were aligned with hisat2.1.0
Counts file is output of hisat
Assembly: GRCh38/hg38
Supplementary files format and content: raw counts in comma-delimited format
|
| |
|
| Submission date |
Apr 22, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Fleur Peters |
| Organization name |
Amsterdam UMC (AMC)
|
| Street address |
Meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE264554 |
CD40 stimulation + dasatinib treatment of chronic lymphocytic leukemia (CLL) cells |
|
| Relations |
| BioSample |
SAMN41046649 |
| SRA |
SRX24335067 |
