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| Status |
Public on Apr 26, 2024 |
| Title |
MicroRNA expression profile in response to CDM-3008 treatment in human hepatocytes. |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Chronic hepatitis B virus (HBV) infections represent a significant global health burden requiring effective therapeutic interventions. This study investigates the antiviral potential of microRNAs (miRNAs) targeting the HBV entry receptor, sodium-taurocholate cotransporting polypeptide (NTCP). Using an interferon (IFN) alpha analog, we highlighted a set of miRNAs induced in treated human hepatocytes. Notably, miR-29b-1-5p was predicted to interact with the 3’-untranslated region (3’-UTR) of NTCP. Functional analysis revealed that miR-29b-1-5p directly targeted and inhibited NTCP. Furthermore, miR-29b-1-5p overexpression significantly reduced HBV genome levels in infected hepatocytes. A rescue experiment demonstrated that miR-29b-1-5p antiviral effect was specifically mediated by NTCP targeting. In summary, these findings underscore the therapeutic potential of miR-29b-1-5p against HBV, advocating for the exploration of miRNA-based therapies in the treatment of human viral infections.
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| Overall design |
Identification of miRNAs induced by the IFN alpha analog CDM-3008 and assessment of their anti-HBV actvity throught NTCP targeting in human hepatocytes.
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| Contributor(s) |
Gailhouste L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 23, 2024 |
| Last update date |
Apr 27, 2024 |
| Contact name |
Luc Gailhouste |
| E-mail(s) |
luc.gailhouste@riken.jp
|
| Organization name |
RIKEN
|
| Department |
Cluster for Pioneering Research
|
| Lab |
Liver Cancer Prevention Research Unit
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| Street address |
408 Main Research Building, 2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1103747 |
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