|
| Status |
Public on Apr 26, 2024 |
| Title |
Control_DMSO_PHH#2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary human hepatocytes (PHH#2), negative control #2, DMSO 8 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human hepatocytes
type: Primary culture
treatment: control
|
| Treatment protocol |
Primary human hepatocytes were treated with 10 µM CDM-3008 for 8 hours. CDM-3008 (RO8191; PubChem CID: 2768133) was from SIGMA and dissolved in DMSO as a 1,000 µM working solution.
|
| Growth protocol |
Human hepatocytes isolated from chimeric mice with humanized livers were purchase from PhoenixBio (Higashi-Hiroshima, Japan) and cultured on collagen type I-coated 96-well cell culture plates in hepatocyte clonal growth medium, as recommended by PhoenixBio.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using a miRNeasy Mini Kit (Qiagen) according to the manufacturer’s recommendations, followed by DNase I treatment (2 units, 37°C for 30 minutes) using a TURBO DNA-free Kit (Invitrogen). RNA concentrations was determined using a DeNovix spectrophotometer. The integrity of the samples was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
miRNA Complete Labeling Reagent and Hyb Kit (Agilent Technologies) according to the manufacturer's instructions. Cyanine-3 (Cy3).
|
| |
|
| Hybridization protocol |
SurePrint G3 Human_miRNA_version 21.0_Microarray (8×60K) and miRNA Complete Labeling Reagent and Hyb Kit (Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
Microarray slides were scanned with an Agilent Technologies G2505C Microarray Scanner.
|
| Data processing |
Raw data were processed using Agilent Feature Extraction software with default parameters to calculate the intensities of the measured spots.
|
| |
|
| Submission date |
Apr 23, 2024 |
| Last update date |
Apr 26, 2024 |
| Contact name |
Luc Gailhouste |
| E-mail(s) |
luc.gailhouste@riken.jp
|
| Organization name |
RIKEN
|
| Department |
Cluster for Pioneering Research
|
| Lab |
Liver Cancer Prevention Research Unit
|
| Street address |
408 Main Research Building, 2-1 Hirosawa
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
| GSE264652 |
MicroRNA expression profile in response to CDM-3008 treatment in human hepatocytes. |
|
