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| Status |
Public on May 13, 2011 |
| Title |
The comparison of gene expression profile in the amygdalae between the wild-type and neuropsin knock-out mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Extracellular proteolysis contributes to fear-associated responses by facilitating neuronal plasticity at the neuron-matrix interface. A serine protease neuropsin is critical for stress-related plasticity in the amygdala by regulating the strength of EphB2/NMDA receptor interaction controlling the expression of fear-related genes and anxiety-like behavior. To look for downstream effects of neuropsin-mediated disassembly of the EphB2/NR1 complex we analyzed gene expression in the amygdalae of wild-type and neuropsin-/- mice.
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| Overall design |
Wild-type control and neuropsin-/- mice were anaesthetised and perfused transcardially with ice cold PBS. The brains were removed and dissected using a vibrating microtome. Amygdalae were dissected from a coronal slice and were stored in RNAlater until processed. The amygdalae have been isolated from 15 brains of neuropsin-/- and 15 wild-type control animals. RNA pulled from 3 animals has been reverse transcribed and hybridized with GeneChip Mouse Exon 1.0 ST Affymetrix Array.
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| Contributor(s) |
Pawlak R |
| Citation(s) |
21508957 |
| Submission date |
Feb 04, 2011 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Marcin Piechota |
| E-mail(s) |
marpiech@if-pan.krakow.pl
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| Phone |
(+4812) 6623328
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| Organization name |
Institute of Pharmacology PAS
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| Department |
Molecular Neuropharmacology
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| Street address |
Smetna 12
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| City |
Krakow |
| ZIP/Postal code |
31-343 |
| Country |
Poland |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA137469 |
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