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| Status |
Public on Jun 18, 2012 |
| Title |
PRMT1 interacts with leukemia fusion protein AML1-ETO for activation of transcription and transformation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Fusion protein AML1-ETO resulted from t(8;21) translocation is highly related to leukemia development. We have previously shown that the expression of AE9a, a spliced form of AML1-ETO, can rapidly cause leukemia in mouse. To understand how AML1-ETO is involved in leukemia development, we used AE9a leukemia model to identify a novel AE9a interacting proteins PRMT1 (protein arginine methyltransferase 1) from primary leukemic cells expressing AE9a. To examine whether PRMT1 is involved in AE9a-mediated transcription regulation, genome wide gene expression analysis is carried out in hematopoietic cell line K562 (wild type or AE9a expressing) treated with (-) control siRNA or siPRMT1.
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| |
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| Overall design |
Wild type or AE9a-expressing K562 cells with control siRNA or siPRMT1 in triplicate
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| |
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| Contributor(s) |
Shia W, Okumura AJ, Yan M, Sarkeshik A, Lo M, Matsuura S, Zhao X, Nimer SD, Yates III JR, Zhang D |
| Citation(s) |
22498736 |
| Submission date |
Mar 23, 2011 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Dong-Er Zhang |
| E-mail(s) |
d7zhang@ucsd.edu
|
| Phone |
858-822-5372
|
| Organization name |
University of California San Diego
|
| Department |
Cancer Center
|
| Lab |
Zhang
|
| Street address |
3855 Health Sciences Drive, Mail Stop 0815
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA139549 |
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