|
| Status |
Public on Jun 18, 2012 |
| Title |
WT_(-)siRNA_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
wild type K562 cells treated with (-) control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: K562
genotype/variation: wild type
sirna: (-) Control siRNA
|
| Treatment protocol |
Cells were nucleofected with 3 μg of siRNA (control or siPRMT1) according to manufacturer’s protocol (Lonza) and harvested after 24 hours.
|
| Growth protocol |
Wild type or AE9a expressing K562 cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini column extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Illumina TotalPrep RNA Amplification Kit from Ambion.
|
| |
|
| Hybridization protocol |
The Illumina hybridization protocol was used. Hybridization in HYB buffer for 18-20 hours at 58°C with gentle rocking followed by standard Illumina washes and streptavidin-Cy3 staining.
|
| Scan protocol |
Illumina BeadArray Reader was used. Green Channel with a PMT setting of 100%.
|
| Description |
Gene expression data from wild type K562 cells treated with (-) control siRNA
|
| Data processing |
Illumina Genome Studio Software produced raw data which was normalized using a modified LOESS.
|
| |
|
| Submission date |
Mar 23, 2011 |
| Last update date |
Jun 18, 2012 |
| Contact name |
Dong-Er Zhang |
| E-mail(s) |
d7zhang@ucsd.edu
|
| Phone |
858-822-5372
|
| Organization name |
University of California San Diego
|
| Department |
Cancer Center
|
| Lab |
Zhang
|
| Street address |
3855 Health Sciences Drive, Mail Stop 0815
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE28135 |
PRMT1 interacts with leukemia fusion protein AML1-ETO for activation of transcription and transformation |
|
