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| Status |
Public on Jan 03, 2012 |
| Title |
Exon array analysis in the mouse myoblast cell line, C2C12 cells treated with control-, Cugbp1- or Mbnl1-siRNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
CUGBP1 and MBNL1 are developmentally regulated RNA-binding proteins that are causally associated with myotonic dystrophy type 1. Using HITS-CLIP anlysis, we found CUGBP1 and MBNL1 preferentially bind to alternatively spliced introns and exons, as well as to the 3' UTRs. To analyze more directly the role of CUGBP1/MBNL1 binding in alternative splicing, we performed exon array analysis in C2C12 cells using expression arrays.
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| |
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| Overall design |
We analyzed total RNA of C2C12 cells treated with control-, Cugbp1- or Mbnl1-siRNA. RNA was harvested 48 hrs after transfection.
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| Contributor(s) |
Masuda A, Ohno K |
| Citation(s) |
22355723 |
| Submission date |
Jun 15, 2011 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Akio Masuda |
| E-mail(s) |
amasuda@med.nagoya-u.ac.jp
|
| Phone |
+81-52-744-2447
|
| Organization name |
Nagoya University Graduate School of Medicine
|
| Lab |
Neurogenetics
|
| Street address |
65 tsurumai-cho, Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA140711 |
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