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| Status |
Public on Feb 01, 2013 |
| Title |
MiR-199a-5p determines fibroblast activation and pulmonary fibrogenesis |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
Idiopathic pulmonary fibrosis (IPF) is a chronic and often fatal pulmonary disorder characterized by fibroblast proliferation and the excess deposit of extracellular matrix proteins. The etiology of IPF is unknown, but a central role for microRNAs (miRNAs), a class of small non-coding regulatory RNAs, has been recently suggested. We report the upregulation of miR-199a-5p in mouse lungs undergoing bleomycin-induced fibrosis and also in human biopsies from IPF patients. Levels of miR-199a-5p were increased selectively in myofibroblasts and putative profibrotic effects of miR-199a-5p were further investigated in cultured lung fibroblasts. MiR-199a-5p expression was induced upon TGFβ exposure and ectopic expression of miR-199a-5p was sufficient to promote the pathogenic activation of pulmonary fibroblasts. CAV1, a critical mediator of pulmonary fibrosis, was established as a bona fide target of miR-199a-5p. Finally, we also found an aberrant expression of miR-199a-5p in mouse models of kidney and liver fibrosis, suggesting that dysregulation of miR-199a-5p represents a general mechanism contributing to the fibrotic process. We propose miR-199a-5p as a major regulator of fibrosis that represents a potential therapeutic target to treat fibroproliferative diseases.
This SuperSeries is composed of the SubSeries listed below.
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| |
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| Overall design |
Refer to individual Series
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| |
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| Contributor(s) |
Mari B, Pottier N, Barbry P, Lebrigand K, Hénaoui I |
| Citation(s) |
23459460 |
| Submission date |
Jan 03, 2012 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platforms (3) |
| GPL7686 |
IPMC - microRNA v7 |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (48)
|
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| This SuperSeries is composed of the following SubSeries: |
| GSE34812 |
miRNA reponse to bleomycin instillation |
| GSE34814 |
mRNA reponse to bleomycin instillation |
| GSE34815 |
Impact of miR-199-5p overexpression and CAV1 silencing on human lung fibroblasts |
|
| Relations |
| BioProject |
PRJNA150247 |
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