|
| Status |
Public on Nov 11, 2005 |
| Title |
Characterization of mRNA transcripts in spermatozoa of fertile men |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by SAGE
|
| Summary |
Accumulated evidence proves that mature spermatozoa contain a complex yet specific mRNA, which could provide information of the past events of spermatogenesis. Our aim was to quantitatively microdissect these mRNA transcripts by a digital approach. SAGE was used to study the mRNA transcripts from ejaculate of an individual and of a pool of 10 fertile men. SAGE library from individual produced 20237 raw tags representing 2459 unique tags (those tags that show at least twice in the library), and that from pooled 10 men generated 21052 raw tags representing 2712 unique tags. The SAGE view of ejaculate of fertile men has revealed a large number of transcripts, which occur in steady frequencies and probably have important implications in the spermatogenesis and fertilization.
Keywords: normal gene expression profile
|
| |
|
| Overall design |
To study the transcripts expression level and their variation in human ejaculate spermatozoa, total RNA extracted from single ejaculate of 10 volunteers were mixed and assigned as “pooled”; for the “individual”, the extracted RNA from 5 separate ejaculate of the same eleventh volunteer were mixed together. And two SAGE libraries were constructed based on these starting RNA materials.
|
| |
|
| Contributor(s) |
Zhao Y, Li Q, Yao C, Wang Z, Zhou Y, Wang Y, Liu L, Wang Y, Wang L, Qiao Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 09, 2005 |
| Last update date |
Apr 18, 2012 |
| Contact name |
Yangxing Zhao |
| E-mail(s) |
yangxingzhao2000@yahoo.com
|
| Phone |
86 21 34201092
|
| Fax |
86 21 54747330
|
| Organization name |
Shanghai Jiao Tong University
|
| Department |
School of Medicine
|
| Street address |
800 Dongchuan Rd.
|
| City |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL4 |
SAGE:10:NlaIII:Homo sapiens |
|
| Samples (2) |
| GSM82243 |
SAGE library from ejaculate sperm of 10 fertile Chinese men |
| GSM82459 |
SAGE library from ejaculate sperm of one fertile Chinese man |
|
| Relations |
| BioProject |
PRJNA93679 |
