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| Status |
Public on May 19, 2012 |
| Title |
Expression data from HEK293T cells infected with Shigella or treated with Rapamycin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Shigella is an intracellular bacterial pathogen known to activate numerous innate immunity and inflammatory signaling pathways. Many of these pathways are also involved in mTOR signaling. We used HEK293T cell cultures and sought to understand how Shigella infection and rapamycin treatment affect the transcriptome and in particular whether there were commonly affected pathways in the two experimental conditions.
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| Overall design |
8 samples were used - 2 control that were uninfected/untreated -- 1 sample lost due to RNA degradation, 3 infected with Shigella for 4 hours, and 3 treated with Rapamycin (50 ug/mL) for 4 hours. All samples were from independent experiments. We used the program AltAnalyze to perform pairwise comparisons between Uninfected with Infected, and Uninfected with Rapamycin using default parameters.
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| Contributor(s) |
Ling A, Girardin S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 18, 2012 |
| Last update date |
Jul 10, 2014 |
| Contact name |
Stephen Girardin |
| E-mail(s) |
stephen.girardin@utoronto.ca
|
| Phone |
1 416 978 7507
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| Organization name |
University of Toronto
|
| Street address |
1, King's College Circle
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| City |
Toronto |
| ZIP/Postal code |
M5S 1A8 |
| Country |
Canada |
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| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA167139 |
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