|
| Status |
Public on May 19, 2012 |
| Title |
HEK293T Shigella infected rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HEK293T
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
treatment: Shigella infected
|
| Treatment protocol |
Shigella infection was done with S. flexneri M90T and a 20 minute room temperature incubation followed by 4 hours of gentamicin medium. Rapamycin treatment was done with similar media and wash steps, but used 50 ug/mL final concentration
|
| Growth protocol |
HEK293T cells were grown in standard tissue culture (DMEM + 5% FBS, pen/strep)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were scraped in cold PBS and were extracted in Trizol.
|
| Label |
biotin
|
| Label protocol |
Protocols were done as per The Ambion WT Expression Kit manual and WT Terminal Labeling Kit (Affymetrix) and were performed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada.
|
| |
|
| Hybridization protocol |
performed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada.
|
| Scan protocol |
performed by The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, Canada.
|
| Data processing |
CEL data was processed with Affymetrix Power Tools (as part of AltAnalyze workflow)
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
RMA normalized probe set expression using APT/AltAnalyze
|
| |
|
| Submission date |
May 18, 2012 |
| Last update date |
May 19, 2012 |
| Contact name |
Stephen Girardin |
| E-mail(s) |
stephen.girardin@utoronto.ca
|
| Phone |
1 416 978 7507
|
| Organization name |
University of Toronto
|
| Street address |
1, King's College Circle
|
| City |
Toronto |
| ZIP/Postal code |
M5S 1A8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE38055 |
Expression data from HEK293T cells infected with Shigella or treated with Rapamycin |
|
