|
| Status |
Public on Jul 09, 2012 |
| Title |
Independence of Repressive Histone Marks and Chromatin Compaction during Senescent Heterochromatic Layer Formation (mRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The expansion of repressive epigenetic marks has been implicated in heterochromatin formation during embryonic development, but the general applicability of this mechanism is unclear. Here we show that nuclear rearrangement of repressive histone marks H3K9me3 and H3K27me3 into non-overlapping structural layers characterizes senescence-associated heterochromatic foci (SAHF) formation in human fibroblasts. However, the global landscape of these repressive marks remains unchanged upon SAHF formation, suggesting that in somatic cells heterochromatin can be formed through the spatial repositioning of pre-existing repressively marked histones. This model is reinforced by the correlation of pre-senescent replication timing with both the subsequent layered structure of SAHFs and the global landscape of the repressive marks, allowing us to integrate microscopic and genomic information. Furthermore, modulation of SAHF structure does not affect the occupancy of these repressive marks nor vice versa. These experiments reveal that high-order heterochromatin formation and epigenetic remodeling of the genome can be discrete events.
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| Overall design |
mRNA expression profiles were compared between normal growing (control) and Ras-induced senescent IMR90 cells. Each includes 5 biological replicates.
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| Contributor(s) |
Narita M, Salama R |
| Citation(s) |
22795131 |
| Submission date |
Jun 01, 2012 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Rafik Salama |
| E-mail(s) |
rafik.salama@ndm.ox.ac.uk
|
| Organization name |
Cancer Research UK
|
| Street address |
Li Ka Shing Centre, Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (10)
|
| GSM941700 |
ER:Ras-IMR90 cells no 4OHT replicate 1 |
| GSM941701 |
ER:Ras-IMR90 cells no 4OHT replicate 2 |
| GSM941702 |
ER:Ras-IMR90 cells no 4OHT replicate 3 |
| GSM941703 |
ER:Ras-IMR90 cells no 4OHT replicate 4 |
| GSM941704 |
ER:Ras-IMR90 cells no 4OHT replicate 5 |
| GSM941705 |
ER:Ras-IMR90 cells 4OHT day 6 replicate 1 |
| GSM941706 |
ER:Ras-IMR90 cells 4OHT day 6 replicate 2 |
| GSM941707 |
ER:Ras-IMR90 cells 4OHT day 6 replicate 3 |
| GSM941708 |
ER:Ras-IMR90 cells 4OHT day 6 replicate 4 |
| GSM941709 |
ER:Ras-IMR90 cells 4OHT day 6 replicate 5 |
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| This SubSeries is part of SuperSeries: |
| GSE38448 |
Independence of Repressive Histone Marks and Chromatin Compaction during Senescent Heterochromatic Layer Formation |
|
| Relations |
| BioProject |
PRJNA167969 |
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