|
| Status |
Public on Jul 09, 2012 |
| Title |
ER:Ras-IMR90 cells no 4OHT replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
IMR90 human diploid fibroblasts, no 4OHT
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
cell line: IMR90
treatment: no 4OHT
transgene: ER:Ras
|
| Treatment protocol |
100 nM 4OHT for 6 days in ER:Ras-expressing IMR90 cells to induced ER:Ras
|
| Growth protocol |
DMEM supplemented with 10% FBS and antibiotics in 5% oxygen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5%O2
|
| Data processing |
The bead-level data were preprocessed using BASH (Cairns et al. (2008) Bioinformatics 24(24):2921-2), a function from the beadarray package (Dunning et al (2007) Bioinformatics 23(16):2183-4) in Bioconductor.
|
| |
|
| Submission date |
Jun 01, 2012 |
| Last update date |
Jul 09, 2012 |
| Contact name |
Rafik Salama |
| E-mail(s) |
rafik.salama@ndm.ox.ac.uk
|
| Organization name |
Cancer Research UK
|
| Street address |
Li Ka Shing Centre, Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE38410 |
Independence of Repressive Histone Marks and Chromatin Compaction during Senescent Heterochromatic Layer Formation (mRNA) |
| GSE38448 |
Independence of Repressive Histone Marks and Chromatin Compaction during Senescent Heterochromatic Layer Formation |
|
