|
| Status |
Public on Sep 21, 2012 |
| Title |
Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
The Arabidopsis thaliana central cell, the companion cell of the egg, undergoes DNA demethylation prior to fertilization, but the targeting preferences and biological significance of this process remain unclear. Here, we show that active DNA demethylation mediated by the DEMETER DNA glycosylase accounts for all of the demethylation in the central cell, and preferentially targets small, AT-rich and nucleosome-depleted transposable elements. The vegetative cell, the companion cell of sperm, also undergoes DEMETER-dependent demethylation of similar sequences, and lack of DEMETER in vegetative cells causes reduced small RNA-directed DNA methylation of transposons in sperm. Our results demonstrate that demethylation in companion cells reinforces transposon methylation in plant gametes, thereby assuring stable silencing of transposable elements across generations
|
| |
|
| Overall design |
Examination of DNA methylation in Arabidopsis endosperm, embryo, and pollen
|
| |
|
| Contributor(s) |
Nishimura T, Zilberman D |
| Citation(s) |
22984074 |
| Submission date |
Jun 26, 2012 |
| Last update date |
Nov 24, 2020 |
| Contact name |
Toshiro Nishimura |
| E-mail(s) |
tnish@berkeley.edu
|
| Phone |
5106429550
|
| Organization name |
University of California at Berkeley
|
| Department |
Plant and Microbial Biology
|
| Lab |
Daniel Zilberman
|
| Street address |
211 Koshland Hall
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
|
| Samples (10)
|
|
| Relations |
| SRA |
SRP013933 |
| BioProject |
PRJNA169395 |
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