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| Status |
Public on Aug 31, 2012 |
| Title |
Reprogramming of DNA methylation in pollen guides epigenetic inheritance via small RNA. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Epigenetic inheritance is more widespread in plants than in mammals, in part because mammals erase epigenetic information each generation by germline reprogramming. To assess the extent of germline reprogramming in plants, we sequenced the methylome of three haploid cell types from developing pollen: the sperm cell (SC), the vegetative cell, and their precursor the post-meiotic microspore.
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| Overall design |
Whole genome bisulfite sequencing of FACS-purified sperm cells, vegetative nuclei and microspores
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| Contributor(s) |
Calarco JP, Borges F, Donoghue MT, Van Ex F, Jullien PE, Lopes T, Gardner R, Berger F, Feijó JA, Becker JD, Martienssen RA |
| Citation(s) |
23000270 |
| Submission date |
Aug 30, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Joseph Calarco |
| E-mail(s) |
jcalarco@cshl.edu
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| Phone |
5163678836
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| Fax |
5163678369
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| Organization name |
Cold Spring Harbor Laboratory
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| Department |
Delbruck Bldg.
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| Lab |
Martienssen
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| Street address |
1 Bungtown Rd
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| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
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| Platforms (2) |
| GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA174185 |
| SRA |
SRP015329 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40501_RAW.tar |
674.5 Mb |
(http)(custom) |
TAR (of GFF) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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