|
| Status |
Public on Jul 01, 2013 |
| Title |
RACK1 function in the miRNA pathway |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
MicroRNAs (miRNAs) regulate plant development by post-transcriptional regulation of target genes. In Arabidopsis thaliana, DCL1 processes precursors (pri-miRNAs) to miRNA duplexes, which associate with AGO1. Additional proteins act in concert with DCL1 (e.g. HYL1 and SERRATE) or AGO1, respectively, to facilitate efficient and precise pri-miRNA processing and miRNA loading. In this study, we show that the
accumulation of plant microRNAs depends on RECEPTOR FOR ACTIVATED C KINASE 1 (RACK1), a scaffold protein found in all higher eukaryotes. miRNA levels are reduced in rack1 mutants and our data suggest that RACK1 affects the microRNA pathway via several distinct mechanisms involving direct interactions with known microRNA factors: RACK1 ensures the accumulation and processing of some pri-miRNAs, directly interacts with SERRATE and is part of an AGO1 complex. As a result, mutations in RACK1 lead to misregulation of miRNA target genes, which is important for ABA responses and phyllotaxy. In conclusion, our study discovered complex functioning of RACK1 proteins in the Arabidopsis miRNA pathway, which are important for miRNA production and therefore plant development.
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| |
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| Overall design |
sequencing of small RNAs from WT and rack1abc mutants (two biological replicates each)
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| |
|
| Contributor(s) |
Speth C, Willing E, Schneeberger K, Laubinger S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Sascha Laubinger |
| E-mail(s) |
sascha.Laubinger@uol.de
|
| Organization name |
University of Oldenburg
|
| Street address |
Carl-von-Ossietzky-Str. 9-11
|
| City |
Oldenburg |
| ZIP/Postal code |
D-26111 |
| Country |
Germany |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA174345 |
| SRA |
SRP015359 |
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