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| Status |
Public on Nov 21, 2012 |
| Title |
Expression profiles of primary motor neurons after knocking down Fus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary motor neurons by using exon-sensitive microarray technology.
To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary glial cells by using exon-sensitive microarray technology.
To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary cerebellar neurons by using exon-sensitive microarray technology.
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| Overall design |
We analyzed total RNA of primary motor neuron infected with lentivirus expressing shRNA against mouse Fus or control. RNA was harvested 11 days after transfection.
We analyzed total RNA of primary glial cells infected with lentivirus expressing shRNA against mouse Fus or control. RNA was harvested 11 days after transfection.
We analyzed total RNA of primary cerebellar neurons infected with lentivirus expressing shRNA against mouse Fus or control. RNA was harvested 9 days after transfection.
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| Contributor(s) |
Fujioka Y, Ishigaki S, Masuda A, Ohno K, Sobue G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 20, 2012 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Shinsuke Ishigaki |
| E-mail(s) |
drshin758@med.nagoya-u.ac.jp
|
| Phone |
+81-52-744-2391
|
| Organization name |
Nagoya University School of Medicine
|
| Department |
Department of Neurology
|
| Street address |
65 Tsurumai-cho, Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
4668550 |
| Country |
Japan |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA181943 |
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