|
| Status |
Public on Jan 23, 2013 |
| Title |
Quality evaluation of Methyl Binding Domain based kits for enrichment DNA-methylation sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Purpose: to compare different Methyl Binding Domain (MBD) based kits for DNA-methylation sequencing using Reduced Representation Bisulfite Sequencing (RRBS) data for validation, and to determine whether data quality can also be derived from inherent sequence data characteristics
|
| |
|
| Overall design |
MBD-seq using 5 different kits (MethylCap, MethylCollector, MethylCollector Ultra, MethylMiner, MethylMagnet) was applied on 3 commonly used cell lines (DU145, HCT15, PC3), for which also RRBS data were generated.
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| |
|
| Contributor(s) |
De Meyer T |
| Citation(s) |
23554971 |
| Submission date |
Dec 06, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Tim De Meyer |
| Organization name |
Ghent University
|
| Department |
Dept. of Mathematical Modelling, Statistics and Bioinformatics
|
| Lab |
BIOBIX lab of Bioinformatics and Computational Genomics
|
| Street address |
Coupure Links 653
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA183137 |
| SRA |
SRP017458 |
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