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| Status |
Public on Oct 01, 2014 |
| Title |
miRNAs were significantly regulated by myocardin and ERα |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Using microarray, we compared miRNAs expression profiles of MDA-MB-231 cells transfected with myocardin and empty vector (pcDNA3.1) and found that 25 miRNAs were significantly changed in myocardin-transfected groups (17 up-regulated and 9 down-regulated miRNAs). Moreover, we showed that 18 of 25 miRNAs significantly regulated by myocardin were inhibited by ERα in MDA-MB-231 cells. In addition,through a microarray approach, we identify the subset of miRNAs modulated by ERα in MDA-MB-231 cells. Our results determined that ERα may function as tumor-promoter through down-regulating expression of 3 miRNAs (miR-26b, miR-146a and miR-331-3p) in MDA-MB-231 cells.
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| Overall design |
There are 4 samples in this experiment (pcDNA3.1-transfected, myocardin-transfected, ERα-transfected, myocardin plus ERα co-transfecteded groups) and each sample was replicated 3 times. The vector-pcDNA3.1 was used as control.
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| Contributor(s) |
Lu D, Wang N, Liao X, Zhang T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 07, 2013 |
| Last update date |
May 02, 2017 |
| Contact name |
da lin lu |
| E-mail(s) |
468891883@qq.com
|
| Organization name |
Wuhan university of science and technology
|
| Street address |
Wuhan university of science and tecnology
|
| City |
Wuhan |
| ZIP/Postal code |
430000 |
| Country |
China |
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| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
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| Samples (12)
|
| GSM1079584 |
breast carcinoma MDA-MB-231 cells were transfected with pcDNA3.1 as control rep 1 |
| GSM1079585 |
breast carcinoma MDA-MB-231 cells were transfected with pcDNA3.1 as control rep 2 |
| GSM1079586 |
breast carcinoma MDA-MB-231 cells were transfected with pcDNA3.1 as control rep 3 |
| GSM1079587 |
breast carcinoma MDA-MB-231 cells were transfected with myocardin rep 1 |
| GSM1079588 |
breast carcinoma MDA-MB-231 cells were transfected with myocardin rep 2 |
| GSM1079589 |
breast carcinoma MDA-MB-231 cells were transfected with myocardin rep 3 |
| GSM1079590 |
breast carcinoma MDA-MB-231 cells were transfected with ERα rep1 |
| GSM1079591 |
breast carcinoma MDA-MB-231 cells were transfected with ERα rep2 |
| GSM1079592 |
breast carcinoma MDA-MB-231 cells were transfected with ERα rep3 |
| GSM1079593 |
breast carcinoma MDA-MB-231 cells were co-transfected with myocardin and ERα rep1 |
| GSM1079594 |
breast carcinoma MDA-MB-231 cells were co-transfected with myocardin and ERα rep2 |
| GSM1079595 |
breast carcinoma MDA-MB-231 cells were co-transfected with myocardin and ERα rep3 |
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| Relations |
| BioProject |
PRJNA188764 |
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