|
| Status |
Public on Oct 01, 2014 |
| Title |
breast carcinoma MDA-MB-231 cells were transfected with pcDNA3.1 as control rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
mammary gland
|
| Organism |
Homo sapiens |
| Characteristics |
transfection: transfected with pcDNA3.1 as control
cell line: MDA-MB-231
|
| Treatment protocol |
MDA-MB-231 breast carcinoma cells were separately transfected with myocardin, ERα，myocardin+ERα and pcDNA3.1
|
| Growth protocol |
Breast carcinoma cells were grown in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% fetal bovine serum at 37℃ in a 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions(QIAGEN miRNAeasy Mini Kit).
|
| Label |
biotin
|
| Label protocol |
Biotinylated microRNA were prepared according to the standard Affymetrix protocol from 1000ng total RNA (Affymetrix® FlashTag™ Biotin HSR RNA Labeling Kits).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug RNA were hybridized for 16 hr at 48C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 7G.
|
| Data processing |
The data were analyzed with PARTEK genomic suit 6.4 using RMA normalization
|
| |
|
| Submission date |
Feb 07, 2013 |
| Last update date |
Oct 01, 2014 |
| Contact name |
da lin lu |
| E-mail(s) |
468891883@qq.com
|
| Organization name |
Wuhan university of science and technology
|
| Street address |
Wuhan university of science and tecnology
|
| City |
Wuhan |
| ZIP/Postal code |
430000 |
| Country |
China |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE44139 |
miRNAs were significantly regulated by myocardin and ERα |
|
