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Status |
Public on Feb 05, 2014 |
Title |
RNA helicase A is necessary for KIF1Bβ tumor suppression in neuroblastoma |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
During development neuronal progenitors compete for growth factors such as nerve growth factor NGF and require the prolyl hydroxylase EglN3 and the kinesin KIF1Bβ for developmental apoptosis. Inherited KIF1Bβ loss-of-function mutations in neuroblastomas and pheochromocytomas implicate KIF1Bβ as a 1p36.2 tumor suppressor, however the mechanism of tumor suppression is unknown. We found that KIF1Bβ interacts with the RNA helicase A (DHX9) resulting in DHX9 nuclear accumulation to regulate apoptosis. KIF1Bβ-dependent DHX9 nuclear localization leads to transcription of the apoptotic target XIAP-associated factor 1. DHX9 is induced when NGF is limiting and required for apoptosis in cells deprived of NGF.
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Overall design |
NB1 cells were transduced to incorporate shRNA against DHX9 or a scrambled control, and transfected with a KIF1Bβ expression vector or control, then transfected cells were isolated and lysed after 48h.
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Contributor(s) |
Chen ZX, Ramskold D, Schlisio S |
Citation(s) |
24469107 |
Submission date |
Feb 22, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Daniel Ramsköld |
E-mail(s) |
daniel.ramskold@ki.se
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Organization name |
Karolinska Institutet
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Lab |
Rickard Sandberg's group (rickard.sandberg@ki.se) (2008-2014, 2018-); Rheumatology unit (2014-2018)
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Street address |
Nobels väg 3
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City |
Stockholm |
ZIP/Postal code |
17177 |
Country |
Sweden |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (12)
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Relations |
SRA |
SRP018815 |
BioProject |
PRJNA190911 |