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Status |
Public on Mar 16, 2013 |
Title |
Amplitude modulation of androgen signaling by c-MYC [RNA-Seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Androgen-stimulated growth of the molecular apocrine breast cancer is mediated by an androgen receptor (AR)-regulated transcriptional program. Through profiling the genomic licalizations of AR and its co-regulators FOXA1 and TCF7L2 in MDA-MB-453 breast cancer cells, we revealed the molecular details of the AR-centered regulatory network. We further identified that c-MYC is a key downstream target co-regulated by AR, FOXA1 and TCF7L2, and reinforces the transctiopnal activation of androgen-responsive genes in this subtype of breast cancers.
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Overall design |
MDA-MB-453 breast cancer cells were transfected with control of MYC siRNA for 48 h, followed by treatment with 10nM DHT or vehicle for 6 h. The cells were subjected to mRNA purification and library praparation for RNA-seq on Illumina HiSeq2000 platform.
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Contributor(s) |
Ni M, Fei T, Chen Y, Brown M |
Citation(s) |
23530127 |
Submission date |
Mar 15, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Yiwen Chen |
E-mail(s) |
ychen26@mdanderson.org
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Organization name |
University of Texas MD Anderson Cancer Center
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Department |
Bioinformatics and Computational Biology
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Lab |
Yiwen Chen
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Street address |
1400 Pressler Street
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (3) |
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This SubSeries is part of SuperSeries: |
GSE45203 |
Amplitude modulation of androgen signaling by c-MYC |
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Relations |
BioProject |
PRJNA193203 |
SRA |
SRP019498 |