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| Status |
Public on May 05, 2014 |
| Title |
An improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-κB as an example |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We provided an improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor. We valided the strategy by characterzing the DNA-binding specificty of NF-κB p50 dimer.
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| |
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| Overall design |
Proteins of the Nf-kappab family were bound to DNA oligonucleotides containing a degenerate region. The protein-DNA complexes were selected after one or multiple rounds of SELEX and the DNA molecules were deep sequenced.
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| Contributor(s) |
Gu G, Wang T, Yang Y, Xu H, Wang J |
| Citation(s) |
24130762, 24992458 |
| Submission date |
Jul 10, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jinke Wang |
| E-mail(s) |
wangjinke@seu.edu.cn
|
| Phone |
86-25-83793620
|
| Organization name |
Southeast Uiniversity
|
| Department |
Biomedicine
|
| Lab |
Wang's Lab
|
| Street address |
Sipailou 2
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210096 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (5)
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| Relations |
| BioProject |
PRJNA210965 |
| SRA |
SRP026681 |
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