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| Status |
Public on Nov 23, 2013 |
| Title |
High-resolution mapping of ribosome footprints from Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Post-transcriptional gene regulation plays a significant role in the response to oxygen deprivation. Here, we utilized advances in next-generation sequencing technology to examine changes in transcriptional control, mRNA loading on to polysome, and regulation of ribosome activity during mRNA translation in 7-day-old Arabidopsis seedlings subjected to 2 hour hypoxia treatment.
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| Overall design |
14 samples, 2 conditions (2 hr hypoxia and 2 hr normoxia), 2 bioreplicates of 3 RNA pools (total mRNA, immunopurified (TRAP) polysomal mRNA, ribosome footprints), 1 bioreplicate of 1 RNA pool (immunopurified (TRAP)-ribosome footprints).
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| Contributor(s) |
Juntawong P, Girke T, Bailey-Serres J |
| Citation(s) |
24367078 |
| Submission date |
Sep 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Piyada Juntawong |
| E-mail(s) |
pjuntawong@gmail.com
|
| Organization name |
Kasetsart University
|
| Department |
Genetics
|
| Lab |
Piyada Juntawong
|
| Street address |
50 Ngamwongwan rd.
|
| City |
Chatujak |
| State/province |
Bangkok |
| ZIP/Postal code |
10900 |
| Country |
Thailand |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA218027 |
| SRA |
SRP029587 |
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