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| Status |
Public on Mar 05, 2014 |
| Title |
A basic helix-loop-helix transcription factors network mediates the brassinosteroid signal [RNA-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand how atypical bHLH, INCREASED LEAF INCLINATION1 (ILI1)-BINDING bHLH-1 (IBH1) (At2g43060), and close homologue, IBH1-like1 (IBL1) (At4g30410), interact to regulate cell elongation, genome-wide RNA-Seq expression analyses of IBH1 and IBL1 gain-(IBH1OE, IBL1OE) and loss-of-function (ibh1 (SALK 049177), ibl1(SALK 119457)) mutants were conducted.
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| Overall design |
For loss-of-function mutant, homozygous ibh1(SALK 049177) and ibl1(SALK 119457) were compared to wild type (Col). For gain-of-function mutant, homozygous 35Spro:IBH1-GFP and 35Spro:IBL1-GFP were compared to wild type (Col). Total RNAs were extactced from seedling of each genotypes. For each genotype two biological replicates were sequenced.
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| Contributor(s) |
Zhiponova MK, Morohashi K, Grotewold E, Russinova E |
| Citation(s) |
24505057 |
| Submission date |
Sep 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kengo Morohashi |
| E-mail(s) |
morohashi.1@osu.edu
|
| Phone |
614-688-4954
|
| Organization name |
The Ohio State University
|
| Department |
Molecular Genetics
|
| Lab |
Erich Grotewold
|
| Street address |
1060 Carmack Rd
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE51121 |
A basic helix-loop-helix transcription factors network mediates the brassinosteroid signal |
|
| Relations |
| BioProject |
PRJNA221230 |
| SRA |
SRP030399 |
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