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| Status |
Public on Mar 01, 2014 |
| Title |
Expression data from U-2 OS osteosarcoma cell lines stably expressing an S22A-progerin mutant compared with U-2 OS expressing wt-progerin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Disassembly of the nuclear envelope is an essential feature of mammalian cell division controlled by the phosphoryaltion of lamins via cyclin dependent kinases. This process is affected in cells expressing progerin, a lamin A allele found in patients with Hutchinson-Gilford Progeria syndrome. Progerin can inhibit cell proliferation of both normal and tumor cells and this property is largely magnified if its phosphorylation at serine 22 is inhibited by a genetic mutation to generate S22A-progerin. Surprisingly, S22A-progerin acquires the ability to trigger cellular senescence in tumor cells with mutations in the p53 and RB tumor suppression pathways suggesting a novel pathway to control the growth of malignant tumors. We used microarrays to characterize the gene expression changes induced by the S22A-progerin in comparison with the wt-progerin in U-2 OS cell line.
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| Overall design |
U-2 OS cells were infected with a retroviral vector that express S22A-progerin or wt-progerin. After 4 days of infection, RNA was extracted from three independents replicas of each condition. Total RNA was send to Genome Quebec service for hybridization with Affymetrix microarrays.
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| Contributor(s) |
Moiseeva O, Lessard F, Acevedo M, Vernier M, Ferbeyre G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 17, 2013 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Mathieu Vernier |
| E-mail(s) |
mathieu.vernier@gmail.com
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| Organization name |
Université de Montréal
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| Department |
Biochemistry
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| Lab |
Dr Ferbeyre
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| Street address |
2900 Edouard Montpetit
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| City |
Montréal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T1J4 |
| Country |
Canada |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA222797 |
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