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Status |
Public on Mar 01, 2014 |
Title |
U2OS-S22AProgerin-1 |
Sample type |
RNA |
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Source name |
U-2 OS_S22A-progerin_4 dpi
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Organism |
Homo sapiens |
Characteristics |
cell line: U-2 OS cell type: osteosarcoma cells infected with: retroviral vector expressing S22A-progerin time point: 4 days post-infection
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Treatment protocol |
Cells were infected with a retrovirus expressing wt-progerin or S22A-progerin and selected during three days with 5 µg/ml puromycin before RNA extraction.
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Growth protocol |
Cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Wisent, Montréal, QC) supplemented with 10% fetal bovine serum (FBS; Wisent) and 1% penicillin G/streptomycin sulfate (Wisent).
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the protocol from the Ambion® WT Expression kit for Affymettrix® GeneChip®Whole Transcrpit (WT) Expression Arrays
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Gene 2.0 ST Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G/4 Color Early Access
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Description |
U2OS_S22A-progerin_1 Gene expression data from U-2 OS infected with a vector expressing S22A-progerin
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Data processing |
The data were analyzed with the affymetrix Expression Console software using Affymetrix default analysis settings and RMA as normalization method. Transcripts with a fold change higher or equal to 2 and a p-value <0.05 according to the two-sample student's t-test were selected for final determination. affymetrix-algorithm-name = rma-gene-full; affymetrix-algorithm-version = 1.0; program-name = Expression Console; program-version = 1.3.1.187
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Submission date |
Oct 17, 2013 |
Last update date |
Mar 01, 2014 |
Contact name |
Mathieu Vernier |
E-mail(s) |
mathieu.vernier@gmail.com
|
Organization name |
Université de Montréal
|
Department |
Biochemistry
|
Lab |
Dr Ferbeyre
|
Street address |
2900 Edouard Montpetit
|
City |
Montréal |
State/province |
Quebec |
ZIP/Postal code |
H3T1J4 |
Country |
Canada |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE51349 |
Expression data from U-2 OS osteosarcoma cell lines stably expressing an S22A-progerin mutant compared with U-2 OS expressing wt-progerin |
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