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| Status |
Public on Mar 01, 2014 |
| Title |
Tissue culture model of hypoxia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Validation of an in vitro model of hypoxia using a primary cell culture of mouse embryonic fibroblasts (MEF) exposed to 1% oxygen at 37 degrees celsius. Control cells were exposed to atmospheric oxygen.
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| Overall design |
Hypoxic and normoxic MEF (in 100 mm dishes, four replicates per group) were submitted to gene expression analysis using Affymetrix Exon microarrays. Differential gene expression analysis was performed with R package DEMI (http://biit.cs.ut.ee/demi/).
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| Contributor(s) |
Ilmjärv S, Luuk H, Hundhal CA, Reimets R, Niitsoo M, Kolde R, Vilo J, Vasar E |
| Citation(s) |
24586062 |
| Submission date |
Jan 21, 2014 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Sten Ilmjärv |
| E-mail(s) |
sten.ilmjarv@gmail.com
|
| Organization name |
University of Tartu
|
| Department |
Institute of Biomedicine and Translational Medicine
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| Lab |
Department of Physiology
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| Street address |
Ravila 19
|
| City |
Tartu |
| State/province |
Tartumaa |
| ZIP/Postal code |
50411 |
| Country |
Estonia |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA235990 |
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