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| Status |
Public on Sep 01, 2014 |
| Title |
ChIP-seq mapping of histone modifications in CD14++ CD16- monocytes from human blood samples |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin immunoprecipitation in combination with a genome-wide analysis via high-throughput sequencing is the state of the art method to gain genome-wide representation of histone modification or transcription factor binding profiles. However, chromatin immunoprecipitation analysis in the context of human experimental samples is limited, especially in the case of blood cells. The typically extremely low yields of precipitated DNA are usually not compatible with library amplification for next generation sequencing. We developed a highly reproducible protocol to present a guideline from the first step of isolating monocytes from a blood sample to analyse the distribution of histone modifications in a genome-wide manner.
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| |
|
| Overall design |
ChIP-seq
histone modifications in CD14++ CD16- monocytes from human blood samples
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| |
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| Contributor(s) |
Bartkuhn M, Weiterer S |
| Citation(s) |
24732314 |
| Submission date |
Feb 03, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Marek Bartkuhn |
| E-mail(s) |
marek.bartkuhn@gen.bio.uni-giessen.de
|
| Organization name |
Justus-Liebig-University Giessen
|
| Department |
Biomedical Informatics and Systems Medicine
|
| Street address |
Aulweg 132
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
|
| Samples (16)
|
| GSM1320312 |
ChIP-seq analysis of H3K27me3 in human monocytes P1 H3K27me3 |
| GSM1320313 |
ChIP-seq analysis of H3K4me3 in human monocytes P1 H3K4me3 |
| GSM1320314 |
ChIP-seq analysis of H3K9Ac in human monocytes P1 H3K9ac |
| GSM1320315 |
Sequencing of input DNA from human monocytes P1 Input |
| GSM1320316 |
ChIP-seq analysis of H3K27me3 in human monocytes P2 H3K27me3 |
| GSM1320317 |
ChIP-seq analysis of H3K4me3 in human monocytes P2 H3K4me3 |
| GSM1320318 |
ChIP-seq analysis of H3K9Ac in human monocytes P2 H3K9Ac |
| GSM1320319 |
Sequencing of input DNA from human monocytes P2 Input |
| GSM1320320 |
ChIP-seq analysis of H3K27me3 in human monocytes P3 H3K27me3 |
| GSM1320321 |
ChIP-seq analysis of H3K4me3 in human monocytes P3_H3K4me3 |
| GSM1320322 |
ChIP-seq analysis of H3K9Ac in human monocytes P3 H3K9Ac |
| GSM1320323 |
Sequencing of input DNA from human monocytes P3 Input |
| GSM1320324 |
ChIP-seq analysis of H3K27me3 in human monocytes P4 H3K27me3 |
| GSM1320325 |
ChIP-seq analysis of H3K4me3 in human monocytes P4 H3K4me3 |
| GSM1320326 |
ChIP-seq analysis of H3K9Ac in human monocytes P4 H3K9Ac |
| GSM1320327 |
Sequencing of input DNA from human monocytes P4 Input |
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| Relations |
| BioProject |
PRJNA237233 |
| SRA |
SRP036159 |
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