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| Status |
Public on Apr 01, 2015 |
| Title |
SKIP Confers Salt Tolerance through Controlling Gene Alternative Splicing in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A better understanding of the mechanisms for plant in response to abiotic stresses is key for the improvement of plant to resistant to the stresses. Much has been known for the regulation of gene expression in response to salt stress at transcriptional level, however, little is known at posttranscriptional level for this response. Recently, we identified that SKIP is a component of spliceosome and is necessary for the regulation of alternative splicing and mRNA maturation of clock genes. In this study, we observed that skip-1 is hypersensitive to salt stress. SKIP is necessary for the alternative splicing and mRNA maturation of several salt tolerance genes, e.g. NHX1, CBL1, P5CS1, RCI2A, and PAT10. Genome-wide analysis reveals that SKIP mediates the alternative splicing of many genes under salt stress condition, most of the new alternative splicing events in skip-1 is intron retention, which leads to the premature termination codon in their mRNA. SKIP also controls the alternative splicing by modulating the recognition or cleavage of 5' and 3' splice donor and acceptor sites under salt stress condition. Therefore, this study addresses a fundamental question on how the mRNA splicing machinery contributes to salt response at a posttranscriptional level.
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| Overall design |
Totally six samples, two treatments and two genotypes, and each have two replicats.
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| Contributor(s) |
Feng J, Gao Z, Ma L |
| Citation(s) |
25617718 |
| Submission date |
Mar 05, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Xu Zhao Gao |
| E-mail(s) |
gaozx@pku.edu.cn
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| Organization name |
Peking University
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| Department |
College of Biological Sciences
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| Lab |
Plant molecular genetics and agricultural biotechnology research laboratory
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| Street address |
The Summer Palace Road No. 5
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA240248 |
| SRA |
SRP039458 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55632_WT-S_vs_WT-0.txt.gz |
288.3 Kb |
(ftp)(http) |
TXT |
| GSE55632_skip-S_vs_WT-S.txt.gz |
290.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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