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| Status |
Public on Dec 01, 2014 |
| Title |
Epidermal RelA specifically restricts contact allergen-induced inflammation and apoptosis in skin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Strong inhibition of NF-kB signaling in the epidermis results in spontaneous skin inflammation in mice and men. Since there is evidence for linkage between polymorphisms within the NF-kB signaling pathway and human inflammatory skin phenotypes, we asked whether partial functional inhibition of NF-kB signaling in epidermal keratinocytes can modulate clinically relevant skin inflammation. We therefore mutated rela specifically in the epidermis of mice (RelAE-MUT mice). These mice show no inflammatory phenotype. Induction of contact allergy, but not croton oil induced irritant dermatitis, resulted in stronger ear swelling and increased epidermal thickness in RelAE-MUT mice. Both contact allergen and croton oil treatment led to increase expression of calgranulins A and B (S100A8/ A9) in RelAE-MUT mice. Epidermal hyperproliferation in RelAE-MUT mice was non-cell autonomous since cultured primary epidermal keratinocytes from RelAE-MUT mice showed reduced proliferation compared to controls. These results demonstrate that epidermal RelA specifically regulates DTH-induced skin inflammation. In addition, we here describe an essential but non- specific function of RelA in the protection of epidermal keratinocytes from apoptosis. Our study identifies new functions of NF-kB signaling in the epidermis and corroborates a specific role of epidermal keratinocytes in the regulation of skin inflammation
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| Overall design |
Mouse ear was treated with 2,4- Dinitro fluorobenzene and collected after 48 h of treatments. Two wild types and two mutant mice were used.
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| Contributor(s) |
Kumari S, Herzberg B, Pofahl R, Krieg T, Haase I |
| Citation(s) |
24739902 |
| Submission date |
Mar 20, 2014 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Snehlata Kumari |
| E-mail(s) |
skumari@uni-koeln.de
|
| Organization name |
University of Cologne
|
| Street address |
Kerpener Strasse 62
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| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA242336 |
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