|
| Status |
Public on Aug 30, 2014 |
| Title |
Virus infection triggers widespread silencing of host genes by a distinct class of endogenous siRNAs in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Virus infection triggers widespread silencing of host genes by a distinct class of endogenous siRNAs in Arabidopsis
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| |
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| Overall design |
This study constructed and sequenced two independent small RNA libraries from the upper uninoculated leaves of (i) WT Arabidopsis plants 14 d after mock inoculation and of (ii) rdr1 rdr6 plants 14 d after infection with Fny CMV-Δ2b, and one library each from the upper uninoculated leaves of (iii) WT, (iv) rdr1, and (v) rdr6 plants 14 d after infection with TuMV-GFP. Coimmunoprecipitation with FLAG- and HA-specific antibodies was used to obtain (vi,vii) AGO1 and (viii,ix) AGO2 complexes, respectively, from the FLAG-AGO1/ago1-36 and HA-AGO2 plants 14 d after infection with Fny CMV-Δ2b for extracting total loaded small RNAs for the construction and sequencing of small RNA libraries.
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| |
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| Contributor(s) |
Ding S, Li Y, Cao M, Wang X |
| Citation(s) |
25201959 |
| Submission date |
Aug 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Peng Du |
| E-mail(s) |
Peng.Du@childrens.harvard.edu
|
| Organization name |
Boston Children’s Hospital, Harvard Medical School
|
| Lab |
Richard Gregory
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
|
| Samples (9)
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| Relations |
| BioProject |
PRJNA259843 |
| SRA |
SRP045894 |
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