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| Status |
Public on Feb 09, 2017 |
| Title |
Role of DLX3 in primary human keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
DLX3 is expressed by differentiated cells in human skin and it has a functional role in epidermal maturation.
We used microarray data analysis to assess gene expression in keratinocytes as consequence of DLX3 expression. We identified a specific subset of genes which are involved in cell cycle regulation and modulate the differentiation program in epidermal cells.
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| Overall design |
Normal human epidermal keratinocytes (NHEK) were isolated from foreskin and maintained in proliferating media. Cells were transfected with a retroviral vector expressing DLX3 (DLx3/Flag). No transduced keratinocytes (KERA) or Empty Vector (VECTOR) transduced cells were considered as controls. RNA samples were collected 24 hours later used for hybridization on Affymetrix microarrays.
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| Contributor(s) |
Palazzo E, Kellett MD, Cataisson C, Gormley A, Bible PW, Pietroni V, Radoja N, Hwang J, Blumemberg M, Yuspa SH, Morasso M |
| Citation(s) |
26522723 |
| Submission date |
Nov 06, 2014 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Hong-wei Sun |
| Organization name |
NIAMS
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| Department |
Office of Science & Technology
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| Lab |
Biodata Mining & Discovery Section
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| Street address |
9000 Rockville Pile
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE67714 |
DLX3-dependent p53 signaling network controls keratinocyte cell cycle and squamous tumor growth |
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| Relations |
| BioProject |
PRJNA266514 |
