|
| Status |
Public on Feb 09, 2017 |
| Title |
NHEK-Control |
| Sample type |
RNA |
| |
|
| Source name |
NHEK_control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary human keratinocytes
|
| Treatment protocol |
Cells were transduced with Empty Vector (VECTOR) or Dlx3/Flag (DLX3) retroviruses (Morasso Lab). No transfected cells were used as control.
|
| Growth protocol |
NHEK were grown in KGM (Keratinocyte Growth Media) (Lonza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from cells was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ng of cDNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data in DLX3-transduced human keratinocytes - Biological replicates
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 06, 2014 |
| Last update date |
Feb 09, 2017 |
| Contact name |
Hong-wei Sun |
| Organization name |
NIAMS
|
| Department |
Office of Science & Technology
|
| Lab |
Biodata Mining & Discovery Section
|
| Street address |
9000 Rockville Pile
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE63049 |
Role of DLX3 in primary human keratinocytes |
| GSE67714 |
DLX3-dependent p53 signaling network controls keratinocyte cell cycle and squamous tumor growth |
|
