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| Status |
Public on Jun 28, 2021 |
| Title |
Gene expression profiles of differentiated Huh7.5.1 cells infected with HCV Jc1 clone |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Hepatocellular carcinoma (HCC) is the second leading cause of cancer death. Curative approaches are limited and chemoprevention is not available. A 186-gene signature in liver tissue predicts HCC risk in cirrhotic patients of various etiologies. However, the drivers of the HCC risk gene signature remain to be fully elucidated. Here, we develop a simple and robust liver cell-based system in which persistent hepatitis B and C virus infection or ethanol exposure induce this signature. We identified candidate drivers of the HCC high-risk signature and hepatocarcinogenesis and uncovered reversal of the signature by an EGFR inhibitor and pioglitazone, a computationally predicted inhibitory compound. This cell-based model unravels the cell circuits of liver disease progression and identifies HCC chemoprevention targets for clinical evaluation.
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| Overall design |
DMSO-differentiated Huh7.5.1 cells were infected with HCV Jc1 clone, and harvested for RNA isolation and gene expression profiling by DNA microarrays.
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| Contributor(s) |
Bandiera S, Venkatesh A, Van Renne N, Sun X, Zeisel MB, Hoshida Y, Baumert TF |
| Citation(s) |
34535664 |
| Submission date |
Mar 12, 2015 |
| Last update date |
Sep 27, 2021 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE66843 |
A cell-based model unravels drivers for hepatocarcinogenesis and targets for clinical chemoprevention |
|
| Relations |
| BioProject |
PRJNA278074 |
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