 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 14, 2015 |
| Title |
Genome-wide chromatin binding of transcription factor E2F1 in castration resistant prostate cancer (CRPC) cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Our computational approach identified E2F1 as a collaborative factor for EZH2 in transcriptional regulation of cancer-related genes. This experiment is designed to validate the interaction between E2F1 and EZH2 on the chromatin. By obtaining over 1 billion bases of sequence from chromatin immunoprecipitated DNA, we generated the genome-wide localizations of E2F1 in CRPC cell line LNCaP-abl cells, and found that Indeed, these sites are enriched near the transcription start sites of EZH2-activated genes. Further analysis of the transcription factor motifs enriched at these peaks revealed the enrichement of androgen receptor motif, suggesting a co-activator role for EZH2 in concert with AR. Our work demonstrated a novel funtion of EZH2 in transcriptional activation by directly binding to the chromatin sites that cooperate with AR.
|
| |
|
| Overall design |
Study of the chromatin localizations of PRC2 complex core subunits and different histone marks in 2 cell types
|
| |
|
| Contributor(s) |
Xu K, Xu H, Chen C |
| Citation(s) |
26659825 |
| Submission date |
Apr 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kexin Xu |
| E-mail(s) |
kxuthscsa@gmail.com
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Molecular Medicine
|
| Street address |
7703 Floyd Curl, MC 8257
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA280968 |
| SRA |
SRP057110 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67809_RAW.tar |
468.6 Mb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
