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| Status |
Public on Apr 18, 2018 |
| Title |
The effect of expression of the Agrobacterium tumefaciens virulence protein VirE3 on gene expression in Arabidopsis thaliana. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During Agrobacterium-mediated transformation of plant cells a part of the tumour inducing plasmid, the T-DNA, is integrated into the host genome. In addition, a number of virulence proteins are translocated into the host cell. The virulence protein VirE3 binds to the Arabidopsis thaliana pBrp protein, a plant-specific general transcription factor of the TFIIB family. To study a possible role of VirE3 in transcriptional regulation, we stably expressed virE3 in A. thaliana under control of a tamoxifen-inducible promoter. By RNA sequencing we showed that upon expression of virE3 the RNA levels of 607 genes were increased more than three-fold and those of 132 genes decreased more than three-fold.
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| Overall design |
An Arabidopsis line expressing virE3 and a control line were selected and the RNA from tamoxifen-induced and non-induced plantlets was analysed by Illumina RNA sequencing.
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| Contributor(s) |
Niu X, van Heusden GP |
| Citation(s) |
26461850 |
| Submission date |
Apr 21, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Paul van Heusden |
| E-mail(s) |
g.p.h.van.heusden@biology.leidenuniv.nl
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| Phone |
31 71 5274996
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| Organization name |
Leiden University
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| Department |
Institute of Biology
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| Street address |
Sylviusweg 72
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| City |
Leiden |
| ZIP/Postal code |
2333BE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| SRA |
SRP057532 |
| BioProject |
PRJNA281883 |
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