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| Status |
Public on Apr 22, 2015 |
| Title |
Methylation Profile of postmature-green-stage seed and dry seed from Arabidopsis ddcc mutant |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The role of non-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the methylome of ddcc mutant in Arabidopsis postmature-green-stage seed and dry seed using Illumina sequencing. Meanwhile, vegetative tissue, leaves from 3 week plant with ddcc mutant and from wild-type, and postmature-green-stage seed and dry seed from wild-type plant were used as control.
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| Overall design |
Illumina sequencing of bisulfite-converted genomic DNA from postmature-green-stage seed, dry seed, and 3-week-plant leaves of ddcc mutant and wild-type.
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| |
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| Contributor(s) |
Goldberg RB, Lin J, Chen M |
| Citation(s) |
29078418 |
| Submission date |
Apr 21, 2015 |
| Last update date |
Jul 19, 2019 |
| Contact name |
Bob Goldberg |
| E-mail(s) |
bobglab@mcdb.ucla.edu
|
| Phone |
310-825-3270
|
| Organization name |
University of California, Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
|
| Street address |
610 Charles E Young Drive East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| SRA |
SRP057531 |
| BioProject |
PRJNA281884 |
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