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| Status |
Public on Apr 23, 2015 |
| Title |
Comprehensive analysis of microRNA expression in the human developing brain reveals microRNA-10 as a caudalizing factor |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human fetal brain. We found that several miRNAs displaying both age-specific and region-specific expression patterns. Among these miRNAs we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while absent from rostral regions. Gain-of-function experiments further demonstrated that miR-10 alone could drive caudalization of human neural progenitors cells. Together, these data confirms a role for miRNAs in establishing different human neural progenitor populations. This data set also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development
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| |
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| Overall design |
MicroRNA sequencing of 34 samples from human embryonic stem cells and human fetal brains.
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| Contributor(s) |
Jönsson ME, Nelander Wahlestedt J, Åkerblom M, Kirkeby A, Jakobsson J, Parmar M |
| Citation(s) |
26395143 |
| Submission date |
Apr 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Malin Parmar |
| Organization name |
Lund University
|
| Department |
Wallenberg Neuroscience Center
|
| Street address |
BMC A11
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA282001 |
| SRA |
SRP057590 |
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