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| Status |
Public on Oct 23, 2016 |
| Title |
Photomorphogenesis of Arabidopsis seedlings is accompanied by complex alternative splicing alterations |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed transcriptome-wide gene expression and AS changes in etiolated Arabidopsis seedlings exposed to red, blue, and white light. Our study revealed that different types of light signals trigger rapid AS responses of numerous genes, including splicing factors and other functional groups. Among these candidates was RRC1, which was previously shown to function in PHYB signaling. The light signaling phenotype of an rrc1 mutant could only be complemented with the splicing variant being up-regulated upon light exposure, indicating a self-reinforcing circuit. Finally, we provide evidence that light-regulated AS can occur in a phytochrome-independent manner and is closely intertwined with the plant’s energy status.
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| Overall design |
Analysis of alternative splicing patterns of dark-grown Arabidopsis seedlings exposed for 1 or 6 hours to white, blue, or red light, or kept in darkness; all samples in duplicates
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| Contributor(s) |
Hartmann L, Drewe P, Rätsch G, Wachter A |
| Citation(s) |
27803310 |
| Submission date |
Jul 07, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Andreas Wachter |
| E-mail(s) |
awachter@zmbp.uni-tuebingen.de
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| Organization name |
University of Tübingen
|
| Department |
Center for Plant Molecular Biology
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| Lab |
Research Group Wachter
|
| Street address |
Auf der Morgenstelle 32
|
| City |
Tübingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA289063 |
| SRA |
SRP060410 |
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