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| Status |
Public on Mar 31, 2016 |
| Title |
Identification of small RNA loci that are synthesized by RNA-dependent RNA polymerases1/2/6 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
To identify RDR1/2/6 dependent small RNA loci of transcribed regions under 2hr drought stress- and untreated conditions, expression profiles of small RNA between rdr1/2/6 and wild-type using hi-seq 2000.
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| Overall design |
rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2weeks. Then, their plants were transfered to dry plastic dish for 2 hours. small RNA fraction was s extracted from the sample using Ambion mirVana miRNA Isolation Kit. small RNA labrary was constructed using TruSeq Small RNA Library Preparation Kits and applied to hi-seq 2000.
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| Contributor(s) |
Matsui A, Mizunashi K, Tanaka M, Iida K, Manabe R, Seki M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 14, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Akihiro Matsui |
| E-mail(s) |
akihiro.matsui@riken.jp
|
| Phone |
+81-45-503-9587
|
| Organization name |
RIKEN
|
| Department |
Center for Sustainable Resource Science
|
| Lab |
Plant Genomic Network Research Team
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| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA295538 |
| SRA |
SRP063657 |
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