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| Status |
Public on Sep 17, 2015 |
| Title |
Small RNA and High Molecular Weight RNA Deep Sequencing Analysis of miR-17~92 Mimics Transfected HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Transient transfection of miRNA mimics are widely used to alter microRNA expression in cells. The goal of this study is to perform small RNA deep sequencing analysis of HeLa cells transfected with miR-17~92 cluster and address potential source of non-specific effects caused by this approach.
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| Overall design |
HeLa cells were cultured as adherent cells. Equal molar concentration of miRNA mimics of miR-17~92 (100nM, 16.7nM each) were transfected and harvedsted at 6h post-transfection for small RNA deep sequencing analysis.
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| Contributor(s) |
Jin H, Sabouri‐Ghomi M, Xiao C |
| Citation(s) |
26697058 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI087634 |
The Role of miR-17~92 in Autoimmune Diseases |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| R01 AI089854 |
Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| R56 AI110403 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| RC1 CA146299 |
Deep Sequencing of Small Regulatory RNAs in Diffuse Large B cell Lymphoma |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
|
| Submission date |
Sep 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Changchun Xiao |
| E-mail(s) |
cxiao@scripps.edu
|
| Phone |
(858) 784-7640
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| Organization name |
The Scripps Research Institute
|
| Department |
Immunology & Microbial Science
|
| Lab |
Changchun Xiao Lab
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA296037 |
| SRA |
SRP063862 |
