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| Status |
Public on Nov 01, 2015 |
| Title |
Rapid and affordable genome-wide bisulfite DNA sequencing by XmaI-RRBS |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We describe XmaI-RRBS method for rapid and affordable genome-wide DNA methylation analysis, with library preparation taking only four days and sequencing possible within four hours. Small sizes of the XmaI-RRBS libraries allow their multiplexing and sequencing on the benchtop high-throughput machines. Described here is the first RRBS protocol validated for the Ion Torrent Personal Genome Machine.
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| Overall design |
DNA from MCF7 cell line and 6 normal breast samples (total 7 samples) were subjected to reduced representation bisulfite sequencing analysis (XmaI-RRBS) by using Ion Torrent platform.
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| Contributor(s) |
Tanas AS, Strelnikov VV |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Alexander S. Tanas |
| Organization name |
Research Centre for Medical Genetics
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| Lab |
Epigenetics
|
| Street address |
Moskvorechie St., 1
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| City |
Moscow |
| ZIP/Postal code |
115478 |
| Country |
Russia |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA299119 |
| SRA |
SRP064958 |
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