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| Status |
Public on Aug 30, 2021 |
| Title |
Deciphering the cell-specific role of Arabidopsis Argonaute 3 [RNA-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
ARGONAUTE (AGO) proteins are the major effectors of RNA silencing. In Arabidopsis, loading of 21-22-nt long small RNAs into AGOs results in post-transcriptional gene silencing (PTGS) by mRNA cleavage and/or translational repression. On the other hand, loading of 24-nt sRNAs results in transcriptional gene silencing (TGS) by RNA directed DNA methylation (RdDM). The Arabidopsis genome encodes 10 AGOs, which are known, for most, to belong to either pathway. Here we characterized the cell specific role of Arabidopsis AGO3. It is specifically expressed in tissue close to vascular termination and strongly accumulates in chalazal seed integuments. AGO3 encodes a functional AGO able to bind sRNAs with a preference for 24nt in length with a 5’ nucleotide bias for Adenosine. Down-regulation of AGO3 affects gene expression in siliques and its expression is strongly induced in the plant vasculature upon proteasome inhibition. Our results suggest that AGO3 might act to regulate gene expression by a novel RNA silencing pathway involving 24nt sRNA-directed PTGS.
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| Overall design |
Transcriptome of Arabidopsis Col-0 Wt and ago3-3 mutant siliques were generated by stranded paired-end deep sequencing, in duplicate, using Illumina HiSeq 2000
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| Contributor(s) |
Jullien PE, Marchais A, Pumplin N, Chevalier C, Schott G, Otto C, Voinnet O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 05, 2015 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Antonin Marchais |
| E-mail(s) |
mantonin@ethz.ch
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| Phone |
+41 44 632 64 08
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| Organization name |
ETHZ
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| Department |
Department of Biology
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| Lab |
Group Prof. Olivier Voinnet
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| Street address |
Universitatstrasse 2
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| City |
Zurich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE74723 |
Deciphering the cell-specific role of Arabidopsis Argonaute 3 |
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| Relations |
| BioProject |
PRJNA301345 |
| SRA |
SRP065841 |
