|
| Status |
Public on Dec 01, 2016 |
| Title |
Next generation sequencing to elucidate the novel function of RORC (RORgamma) in breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Exploring the novel role of RORC (RORgamma) in breast cancer, utilizing NEXTseq with genetic gain and loss of function and pharmacological treatment.
|
| |
|
| Overall design |
For loss of function, control-siRNA or RORC-siRNA was transfected for 48h in three cell lines (MCF-7, T-47D and MDA-MB-231). For gain of function, CMV-empty or CMV-RORC was transfected for 48h in MDA-MB-231 cells. Furthermore, the selective RORC antagonist, SR2211 was utilized. MCF-7 cells were treated either DMSO or SR2211 (5uM) for 24h. Total RNA was extracted with the RNeasy kit. NEXTseq was performed for transcriptome analysis.
|
| |
|
| Contributor(s) |
Oh TG, Muscat GE |
| Citation(s) |
27211549 |
| Submission date |
Nov 13, 2015 |
| Last update date |
May 27, 2020 |
| Contact name |
Tae Gyu Oh |
| E-mail(s) |
t.oh@uq.edu.au
|
| Organization name |
Institute for Molecular Bioscience
|
| Street address |
306 Carmody Rd
|
| City |
St Lucia |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (30)
|
|
| Relations |
| BioProject |
PRJNA302118 |
| SRA |
SRP066204 |
Less...
More...