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| Status |
Public on Nov 23, 2016 |
| Title |
A faithful in vivo model of human MLL-AF4 proB acute lymphoblastic leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome analysis by RNAseq of leukemia model promoted by MLL-Af4 or MLL-AF9 fusion proteins. We find each fusion protein promotes a specific gene signature correlating to those identified in patients
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| |
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| Overall design |
Human CD34+ hematopoietic stem and progenitor cells were transduced with retrovirus expressing MLL-Af4 or MLL-AF9. Transduced cells were transplanted into immunodeficient mice to induce lymphoid leukemia or placed in myeloid in vitro culture. CD19+ lymphoid leukemia cells (3 AF9, 6 Af4), control health CD19+CD34+ proB cells (n=3) and 4 pairs of Af4 and AF9 CD33+CD19- myeloid culture cells were collected for RNA-seq
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| |
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| Contributor(s) |
Lin S, Mulloy JC, Luo RT, Thirman MJ |
| Citation(s) |
27846391 |
| Submission date |
Jan 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Shan Lin |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
EHCB
|
| Lab |
James Mulloy
|
| Street address |
3333 Burnet Avenue, CCHMC
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA309171 |
| SRA |
SRP068584 |
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